Mode of action / characterization

• Viability (MTT, LDH, FACS)

• Cell adhesion, cell proliferation (cell counter, trypan blue exclusion, FACS)

• Cell Metabolism :  a unique platform for subrates and enzymatic activity measurement (Citrate synthase, complex I-V,  SOD,  GS, GCS…)

• Oxidative stress (NO, iNOS, …)

• Cartilage catabolism and anabolism : COLL2.1, COLL1, ACAN, MMP, ADAMTS (qPCR, IF / IHC, WB, ELISA, Multiplex)

• Inflammation : cytokines (WB, ELISA, Multiplex)

Characterization by Omics : genomics (DNA), transcriptomics, proteomics, and metabolomics