In vitro test systems

• Primary cells : synoviocytes, chondrocytes, osteoblast, myoblast, fibroblast, macrophage, Peripheral blood mononucleated cells , adipocytes, Mesenchymal stromal/stem cells  (from different sources)
​   - Human (biopsy from healthy or diseased volunteer), rat, mice, dog, sheep, rabbit, bovine, horse

• Cell lines :  RAW264.7 , L-929, Caco-2, HT29-MTX, NIH3T3,…
   -  Human, rat, dog, sheep, rabbit, bovine, horse

• Control or stimulated conditions (IL-1β , TNFα, LPS, IFNγ)

• Differentiation : trilineage differentiation test (osteoblast, chondrocyte, adipocyte), M1/M2 polarization (macrophage)

• Monolayer, 3D and organoid cultures

• Direct or indirect contact (insert)

• Collection of cells and supernatants for further OMICS analysis

  → Cytotoxicity study, immunogenicity study, mechanism of action and characterization             of compounds